In recent research, an investigation has been led to search for antibiogram patterns from an alternate natural source, endophytic fungus. The study has revealed the presence of good antibacterial activity for the crude extracts of Penicillium sp. so. This means that this organism could be a good source for bioactive compounds, and the isolated compounds can be further used as an antibacterial agent.
In detail, the study was aimed to screen the antimicrobial potential of 6 endophytic fungi isolated from leaves of the Centella Asiatica plant. The endophytes were isolated using two different mycological media, namely Potato Dextrose Agar (PDA) and Malt Extract Agar (MEA). Maximum endophytes were isolated in PDA. The ethyl acetate extract of endophytic fungi showed growth inhibition on at least one pathogenic bacteria Klebsiella sp., Staphylococcus sp., Pseudomonas sp., Salmonella sp., Proteus sp., Shigella sp., Serratia sp. But the activity against fungal pathogens was very low compared to bacteria.
The extracts’ Minimum inhibitory concentration (MIC) values ranged from 50.6µg/ml to 274.6µg/ml. Among all isolated endophytic fungi isolated, the fungus with the highest positive antimicrobial activity was identified as Penicillium sp. by 18S ribosome RNA sequence analysis. The sequence was submitted to NCBI, and the accession number HM068965. The results indicated that the endophytic fungi of medicinal plants could be promising sources for bioactive compounds. The purification and determination of bioactive compounds are needed to perform a further study.